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Postiosoite: PL 66
00014 Helsingin yliopisto
Käyntiosoite:
Agnes Sjöbergin katu 2
Puhelinnumero: 09 1911 (vaihde)
Faksi: 09 191 57161
Sähköposti:
eltdk-hallinto@helsinki.fi

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Alma-intranet

BD FACSAria II Cell Sorter at Division of Veterinary Biosciences


The cell sorter is located at the EE building (Agnes Sjöbergin katu 2), 4th floor, lab 4091. It can be used for high-performance sorting of cells and other particles.
For flow cytometry analysis without sorting, we recommend using an analytical flow cytometer. See http://www.helsinki.fi/biosciences/biochemistry/flowcytometry.htm for an analytical instrument in Viikki.

Lasers and detectors available

Please see the current specifications here (.pdf).

How to get started with the instrument

The instrument can be used by trained personnel. Documented earlier use of a cell sorter or user training for sorting provided by BD or other expert institution are valid qualifications. We can provide training to upgrade the instrumentation skills for researchers who have practical experience of using an analytical instrument. Other training options and assisted sorting are negotiable.
Please contact the FACSAria administration by sending an email to facsaria-admin [ät] helsinki.fi. To apply for access to the instrument, specify the following details:

  • Your name, email, phone number and work address
  • Your 8-letter username in the university IT system
  • Billing address and contact details for the person who will pay the instrument usage fees
  • What kind of samples you are planning to sort

We will create for you a user account for booking and using the instrument.


Fees

Helsinki University users are charged 30€/hour, and other users are charged 60€/hour, based on the user log of the FACSDiva software (starting when you log in the software and ending when you log out). The fees for user training per hour will consist of the instrument charge and a teaching fee (40 e/h).
Basic solutions (FACS Flow etc.) and basic disposables (test tubes, cell strainers, CS&T beads) are provided.

Booking the instrument

The booking system is available at: https://varaus.it.helsinki.fi/day.php?area=54
Log in using your Helsinki university account (note that this is possible only when we have activated your account). Select the correct day, then click the + symbol to add a booking. Specify the details (starting time, duration and a brief description about what type of samples you are running). Finally click SAVE.

You may have a maximum of three bookings in the system.

Actually using the instrument

You are required to master the instrument to be allowed to use it. We will create a FACSDiva user account for you. For your convenience, brief operating instructions are provided here in English (.pdf). Please also note our rules for FACSARia users (.pdf). We will also provide all users practical guidance for working in of the flow lab before start.

The FACSAria users' mailing list


To receive notifications concerning FACSAria users, subscribe to our users' mailing list by sending an email to majordomo@helsinki.fi, with no subject, and "subscribe facsaria-viikki [ät] helsinki.fi" as the message body.

The FACSAria team

Technical staff (buffers, disposables):

  • Kati Holmsten

Academic staff (administration):

  • Sami Junnikkala
  • Tiina Pessa-Morikawa (user training and consultation)


Page updated: 07.09.2012